FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_SAL180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_SAL180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94467271
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG99250.029.2417075
GTCTAGG264950.023.2119391
TATACCG271250.022.6248025
GTACCGT146050.022.5709196
CTTCTGC2118000.021.3063794-95
TTCTGCT1606650.021.01393594-95
CCTAGAC275150.020.9924413
AGTACCG209500.020.6323835
TACCGTG184800.020.1508757
GGACTGC192450.019.6959236
ACTGAGC307000.019.6492148
ACTGTCC147200.019.3607988
CACTGAG338450.019.2828877
TCTTCTG2846800.019.23072894-95
GTCTATG306300.019.2249571
CTAGACA280750.019.1522524
GTATAAG458450.018.9092331
GTCTTCT3434450.018.80812894-95
GTGTATG350100.018.801771
TAGAGCG241450.018.6300835
Produced by FastQC (version 0.11.5)