FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85721783
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG106100.033.5767065
GTCTAGG255150.024.0853581
TATACCG296700.023.1975985
GACGGTC138900.022.4668737
ATAGACG134200.022.440474
CCTAGAC259050.022.297253
GTCTATG323850.021.2654511
CTAGACA329800.020.898354
TAGAGCG268400.020.8475465
GTACCGT182350.020.5263566
CTTCTGC1717300.019.6834694-95
CACTGAG424800.019.2990467
AGTACCG255050.019.2383215
TTCTGCT1283600.019.09922894-95
GCCCTAG201450.018.9451661
TCTTCTG2249800.018.92224994-95
GTCTTCT2705550.018.65807394-95
GTGTATG378150.018.6391961
ACTGAGC401200.018.6110618
ACTGTCC172200.018.5359848
Produced by FastQC (version 0.11.5)