FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164383202
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT7297750.026.1170194-95
TCTGCTT5074950.024.50715894-95
TAGACGG145400.024.501275
CTTCTGC10204000.024.43658394-95
GTACCGT230350.021.7754276
TATACCG386000.021.768695
GTCTAGG374000.021.730291
TCTTCTG13786700.021.51616994-95
CTGCTTG3139800.021.11561494-95
ACTGTCC223700.020.6810198
TACCGTG308100.019.6715227
TAGAGCG391050.019.3497455
GTCTTCT16899100.019.02367294-95
AGTACCG339000.018.9298615
CCTAGAC420650.018.2256373
ACTGAGC512350.018.1983728
CACTGAG538600.018.1668577
GTCTATG444400.017.785221
GGACTGC296800.017.5883396
GTATAAG620600.017.4071921
Produced by FastQC (version 0.11.5)