FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60185424
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG171200.016.0642745
CCGTCTT1073050.015.63991994-95
GCCGTCT1029850.014.72772894-95
GTCTAGG161500.014.5945361
CCTAGAC159400.013.7970133
TAGAGCG172000.013.7802925
TTCCGTC940900.013.744699594-95
TCCGTCT679500.013.73325294-95
TAGACGG88950.013.6169715
TGCCGTC1602450.013.52474894-95
CCTATAC289750.013.508163
ACTGTCC143900.013.40131958
TATACTG246650.013.2685785
GTACCGT142950.013.2578976
CTATACT304950.013.1929684
ACTGAGC276450.012.9893528
AGTACCG175000.012.5940735
CACTGAG262800.012.5615077
CGTCTTC1010100.012.41275394-95
GTCTATG202850.012.205171
Produced by FastQC (version 0.11.5)