FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65439295
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2328600.020.48096894-95
TCTGCTT1791150.020.3809194-95
CTGCTTG1272950.018.56120394-95
CTTCTGC2939650.018.49081294-95
TGCTTGA955000.017.60308894-95
GCTTGAA739200.016.95535394-95
TCTTCTG3707700.016.80704394-95
GTCTTCT4421200.016.4326294-95
TATACCG167500.016.2208215
CGTCTTC5469000.015.55778994-95
CCGTCTT6380500.015.31669894-95
GCCGTCT7682900.014.54724494-95
GTATAGG273350.014.2225271
CTATACT290750.013.8211414
CCTAGAC153650.013.4478943
CCTATAC282200.013.2132673
TGCCGTC8984050.013.20520194-95
GTCTAGG156450.013.0020171
TAGACGG84250.012.9672995
ACTGAGC233000.012.9448638