FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98136397
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG120100.030.0976335
GTCTAGG276050.024.5508541
TATACCG289150.022.0455045
CCTAGAC296350.021.0294763
GTACCGT173700.020.0991656
TAGAGCG261250.019.9816845
ACTGTCC174050.019.8945548
AGTACCG251950.019.324145
ACTGAGC375300.019.2753418
CACTGAG403800.019.1735297
ATAGACG157350.019.1086524
GTCTATG334050.019.0361631
GTGTATG385300.018.909411
GGACTGC226650.018.6938886
CTTCTGC1706100.018.66563694-95
GACGGTC168950.018.6114657
TACACCG217050.018.1638955
TATACTG423000.017.8881785
GTATAAG486650.017.7937091
GTCTTCT2565800.017.48515594-95
Produced by FastQC (version 0.11.5)