FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_NON15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_NON15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179745943
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG181650.027.9794625
TTCTGCT6700200.024.70629794-95
TATACCG498200.023.2445415
TCTGCTT4835000.023.22830294-95
CTTCTGC9035250.022.44253394-95
GTCTAGG446150.022.3171231
GTACCGT287850.021.336476
CTGCTTG3118550.019.96389894-95
ACTGTCC287550.019.8385148
TAGAGCG432400.019.8281655
TCTTCTG11741750.019.63269294-95
AGTACCG411000.019.42755
TACCGTG375300.018.7690417
GTCTATG545450.018.6724931
CTAGACA513950.017.7726354
TACACCG347950.017.6374865
GTCTTCT14160350.017.61788694-95
GGACTGC369650.017.5786556
CCTAGAC474050.017.485183
ACTGAGC624400.017.2832328
Produced by FastQC (version 0.11.5)