FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141712395
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG147650.028.3099255
TTCTGCT4242150.025.76702994-95
CTTCTGC5954500.023.76711894-95
GTACCGT224550.023.6704436
TCTGCTT3017050.023.1249294-95
TATACCG403850.022.9705645
GTCTAGG379800.021.8361851
AGTACCG322750.021.354565
TCTTCTG7826300.020.90665694-95
TACCGTG288550.020.477547
ACTGTCC222200.020.2434448
GTCTATG439400.020.0641231
TAGAGCG379500.020.0386775
CTAGACA426000.018.9441834
CACTGAG557150.018.9345467
ACTGAGC497500.018.6556328
CTGCTTG1885150.018.65168494-95
GACAGTG247800.018.4779687
CCTAGAC403800.018.2567253
GTCTTCT9445000.018.24476694-95
Produced by FastQC (version 0.11.5)