FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255588
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTC352.7840017E-454.2850465
GGCATCC752.3737812E-950.6660427
ATCCCGT652.581657E-643.845616
GTTACCA459.61159E-442.2217039
ATACCGC459.61159E-442.2217036
GTAACAC459.61159E-442.2217033
GCATCCC802.2536005E-741.5619858
GGCTTAG608.244671E-539.606091
AGGCATC756.901775E-637.999536
TACACGG500.001613562837.999535
CTTAGGC1152.4865585E-937.1734543
CTGAGCG550.00257604534.545039
TGGCCTA550.00257604534.545035
GTGTATG702.0419725E-433.9480821
CTAGACA702.0490307E-433.9281544
GTGCAAT600.003934165531.6848741
GCCCTAG753.061458E-431.6848721
GCTAGAG753.061458E-431.6848722
GGTGTCC753.0720243E-431.6662778
GTGGGTC753.0720243E-431.6662774
Produced by FastQC (version 0.11.5)