FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71724449
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2003750.013.07297994-95
CCGTCTT1566150.012.97839294-95
TATACTG210950.012.78965955
TGCCGTC2450700.012.28399994-95
GTATTGG443850.012.0892471
ATGCCGT2430500.011.86133394-95
TATGCCG2585050.011.53284292-93
TTCTGCT911050.011.52290694-95
CCTAGAC212300.011.4556283
GTCTATG213550.011.3949371
TATACCG174000.011.3016345
TCTGCTT845550.011.20767694-95
GTATAGG206550.011.15984251
CTATACT252650.010.9795834
GTCTAGG226950.010.9734351
GTATAGA206750.010.9191691
TAGACTG142450.010.6036375
GTATGCC2782600.010.3854792-93
GTATATG288350.010.3839391
TACACAG176550.010.3312695
Produced by FastQC (version 0.11.5)