FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102431994
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG136800.027.1178235
GTCTAGG315150.022.6520521
GTCTATG361150.021.8198551
TATACCG307400.021.52475
TAGAGCG347300.020.0638945
CCTAGAC302100.019.780123
ATAGACG157000.019.726034
GTACCGT257000.019.6466886
ACTGTCC255950.019.5412488
ACTGAGC597050.019.1171058
CACTGAG651000.019.0723277
AGTACCG320650.019.0650165
AACAGAC322900.018.9464077
GTATAAG426850.018.4613821
GACAGTG330350.017.9152477
TATACTG403400.017.7446425
GTGTATG395650.017.718861
CTAGACA370650.017.6338124
GTCTAGA283150.016.97031
GTCTTCT2147950.016.86943294-95
Produced by FastQC (version 0.11.5)