FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121459692
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3559500.025.3292794-95
TAGACGG123150.024.5309055
CTTCTGC5016850.023.63070794-95
GTCTAGG319200.022.6913661
TCTGCTT2534400.022.53945594-95
GTACCGT220400.022.2843326
TATACCG307750.022.1485185
GTCTATG370600.021.0446511
TCTTCTG6518150.020.84685794-95
AGTACCG303050.020.2663295
ACTGTCC214200.019.7799578
GACAGTG243100.019.2846667
TACCGTG265150.019.2752747
ACTGAGC523400.019.1482328
CACTGAG554500.019.02517
TAGAGCG339050.019.0250975
GTGTATG381050.018.7213541
CCTAGAC326650.018.4388983
CTGCTTG1604800.018.33287894-95
GTATAAG470050.018.2705941