FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83016180
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG118850.024.0995335
GTCTAGG279550.022.7643071
GTCTATG342350.020.6291731
CCTAGAC259200.019.1504233
TATACCG269200.019.1269075
ACTGTCC278400.018.5114128
AACAGAC294650.018.1997977
TAGAGCG360600.018.033015
ATAGACG134500.018.0110784
TATACTG323750.017.3126185
CTAGACA317350.017.122984
GACAGTG315600.016.6906627
GTGTATG351400.016.270361
GTATAAG327450.016.12511
TACACCG217700.016.0150455
CACTGAG688800.015.6604067
ACTGAGC646300.015.433498
ACAGACC362350.015.3368658
GTACCGT251100.015.3224626
GTATAGG337900.015.2325861
Produced by FastQC (version 0.11.5)