FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_EXW15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_EXW15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125247834
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3699550.024.8556394-95
CTTCTGC5137100.022.93087494-95
TCTGCTT2651650.022.53514794-95
TATACCG300650.021.2655325
GTACCGT211650.020.9165086
GTCTAGG319600.020.74471
TCTTCTG6704800.020.43574194-95
ACTGTCC211950.019.7209648
AGTACCG283300.019.2983935
TACCGTG267100.018.6721487
GTCTATG364950.018.5054821
CCTAGAC338900.018.4873053
TAGAGCG331950.018.3588895
CTAGACA326900.018.3519784
GTATAAG470600.018.239191
GTCTTCT8115600.018.2013794-95
CTGCTTG1713350.018.1264294-95
TAGACGG175350.017.7159465
ACTGAGC484450.017.4325798
GTGTATG406000.017.2080251
Produced by FastQC (version 0.11.5)