FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68660140
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14578532.1232887087034777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA1097000.020.01762294-95
TATCATT1597300.018.01628394-95
GTATCAT1886600.016.96492294-95
ATCATTA1593850.016.68199394-95
CGTATCA2217750.015.62851394-95
ATGCCGT77600.014.6305454
CCGTATC2570650.014.49240294-95
TAGACGG57600.013.9373525
GTACCGT69650.013.7085746
CATTAAA1143900.013.70762394-95
AGTACCG86450.013.6270795
TACCGTG83400.013.3844777
GCCGTAT3085500.013.35292694-95
GTATAGG141650.013.0879881
GTCTATG133900.012.567461
CGCCGTA3644550.012.55336894-95
CACTGAG141750.012.3987597
GTCTTAG102600.012.3242091
GGACCGT81550.012.0576796
TACACCG94900.011.8130455
Produced by FastQC (version 0.11.5)