FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_SAL60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_SAL60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103239169
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3817400.024.11787694-95
CATTAAA2703500.022.9642194-95
ATCATTA5053750.022.77561894-95
TAGACGG107800.020.7971085
TATCATT6292100.020.43819694-95
TATACCG256450.020.4292555
GTCTAGG270850.019.510331
GTACCGT146950.018.1977946
GTATCAT7580950.018.17616794-95
GTCTATG309000.017.0400141
TACCGTG188250.016.703137
AGTACCG204950.016.3620035
CGTATCA9090950.015.85725494-95
TAGAGCG242850.015.7056955
ACTGTCC156300.015.619898
GGACTGC201100.015.5887686
GGACCGT198500.015.5058096
ACTGAGC310550.015.493588
CCTAGAC270950.015.47940353
GTATAAG435200.015.4509861
Produced by FastQC (version 0.11.5)