FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_SAL15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_SAL15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69123653
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2637700.024.2448694-95
CATTAAA1840050.023.2223994-95
ATCATTA3464350.023.03633594-95
TAGACGG70500.022.4356465
TATCATT4354850.020.48102494-95
TATACCG177500.020.4445935
GTACCGT94200.019.9677146
GTATCAT5232000.018.1930894-95
GTCTAGG184000.018.181881
AGTACCG138050.018.0292785
TACCGTG123600.017.6774027
AAGACCG145300.016.6720125
GGACTGC130800.016.6682156
GTCTATG210250.016.386491
GTATAAG310200.016.3151761
CTAGACA189200.016.1426074
TAGAGCG164900.016.0441995
CCTAGAC185450.015.9312153
GTGTATG237650.015.7169651
CACTGAG222800.015.6906787
Produced by FastQC (version 0.11.5)