FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_POK60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_POK60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87070649
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG89900.023.9870415
TCATTAA2805750.022.69938994-95
ATCATTA3640150.021.98426894-95
CATTAAA2022300.021.34311794-95
GTACCGT136500.021.2960766
GTCTAGG227250.021.2668631
TATACCG237950.021.0594645
TATCATT4515050.019.91983294-95
AGTACCG189250.019.3006085
TACCGTG173950.018.75897
TAGAGCG215550.018.6424475
CCTAGAC232300.018.463833
GTATCAT5385700.017.96474894-95
GGACTGC174750.017.8850236
CACTGAG297550.017.8147327
CTAGACA241300.017.7160244
ACTGTCC138750.017.4586948
GTCTATG274650.017.458141
ACTGAGC291250.017.2378718
GTATAAG375000.016.8288021
Produced by FastQC (version 0.11.5)