FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_POK5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_POK5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83013270
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA1604100.016.4800894-95
CCGTATC3957350.016.08613494-95
TCATTAA1873150.015.85885794-95
GCCGTAT5072300.015.74723594-95
CGTATCA3250850.015.68452894-95
CGCCGTA6221800.015.24917894-95
GTATCAT2781250.013.9308394-95
TATACCG246100.013.7613545
ATCATTA2134350.012.70295994-95
TAGACGG122850.012.4498465
GTATAGG361650.012.3383521
TCGCCGT7524650.012.18940594-95
TATCATT2393600.011.85197292-93
ATTAAAA1629800.011.84420694-95
TAGAGCG258500.011.7598755
CCTAGAC225300.011.7008993
GTCTAGG221350.011.3568051
TAGAGGT307250.011.2235134
ACTGTCC200900.010.9466238
TTAAAAA1455300.010.84423694-95
Produced by FastQC (version 0.11.5)