FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_POK180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_POK180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87271493
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG96250.023.9837635
GTCTAGG231400.020.5361371
TATACCG234150.020.4478725
ATCATTA2356200.019.99477294-95
TCATTAA1885100.019.15725394-95
TATCATT2908300.019.11515294-95
GTATCAT3470800.018.74264794-95
CGTATCA4228800.017.75420494-95
GTACCGT144850.017.4779596
ACTGTCC144200.017.1941818
CATTAAA1409250.017.09855894-95
CCTAGAC231100.017.0387553
CCGTATC5149550.016.97838494-95
AGTACCG191750.016.819625
ACTGAGC294300.016.7687858
GTCTATG272450.016.5000951
CACTGAG305100.016.4709747
GTATAAG378800.016.3838251
CTAGACA256200.016.314974
TACCGTG178700.016.29347
Produced by FastQC (version 0.11.5)