FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_POK15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_POK15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106947007
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA5580600.025.93912994-95
CATTAAA3824500.025.5007394-95
ATCATTA7670150.024.74735394-95
TAGACGG91700.023.050095
TATACCG216600.022.5432555
TATCATT9882750.022.38084894-95
GTACCGT136550.022.0188486
GTCTAGG241300.021.2100281
CTAGACA223350.020.1181184
AGTACCG193600.020.0201245
TACCGTG173550.019.5685737
GTATCAT12341600.019.47403394-95
ACTGTCC137250.019.1722958
TAGAGCG227300.019.0788975
TACACCG164650.018.7513945
GTCTATG252550.018.5717411
GTATAAG355600.018.0006711
CTAGACG101100.017.9471044
GGACCGT187000.017.8818976
GGACTGC178550.017.8502886
Produced by FastQC (version 0.11.5)