FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108002092
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG108100.027.7258455
TCATTAA3334250.022.778994-95
GTACCGT150300.022.2482096
ATCATTA4272900.021.98245894-95
GTCTAGG307100.021.7255211
CATTAAA2340600.021.592294-95
TATACCG320200.021.1385255
TATCATT5230100.019.48775194-95
GACGGTC134650.019.1896767
AGTACCG234450.018.861695
GTCTATG361250.018.62681
ACTGTCC153200.018.5713548
GGACTGC213050.018.2592836
CCTAGAC322650.018.1958083
CCGTGCG165350.018.1545459
TACCGTG203600.017.7067627
GTATCAT6141050.017.47099594-95
CTAGACA319650.017.4303154
TAGAGCG275950.017.3504715
GTGTATG396300.017.1712471
Produced by FastQC (version 0.11.5)