FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123895532
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG119800.025.3748445
TCATTAA4367250.024.67588894-95
ATCATTA5826300.023.46498594-95
CATTAAA3029450.023.36968494-95
GTACCGT175400.022.4223616
TATACCG327850.021.5295
TATCATT7318650.020.93132894-95
GTCTAGG324100.020.2635861
ACTGTCC177500.019.7752488
TACCGTG222700.019.5793617
AGTACCG260150.018.8241375
GTATCAT8835600.018.5878794-95
TAGAGCG304300.017.997315
GGACTGC235400.017.9784346
CTAGACA339650.017.8443054
GTCTATG376100.017.8409771
CCTAGAC329100.017.3195633
CACTGAG408900.017.1569237
GACGGTC149350.017.0147117
AAGACCG269550.016.9694275
Produced by FastQC (version 0.11.5)