FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52666310
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2405350.015.80288294-95
GCCGTAT3058050.015.479794594-95
CATTAAA1091000.015.04875994-95
CGTATCA1992000.014.95446894-95
CGCCGTA3667750.014.622498594-95
TCATTAA1251500.014.11700794-95
TATACCG160850.014.0857385
TAGAGCG154150.013.6194945
GTCTAGG158750.013.2903591
GTATCAT1732150.012.94056894-95
TAGACGG74950.012.4846645
ATCATTA1399100.011.53959292-93
GTCTATG186250.011.5321311
TCGCCGT4376650.011.43139394-95
CCTAGAC156650.011.2190183
TATACTG241850.011.1161055
ACTGAGC233400.010.9486468
CACTGAG239950.010.9071037
TATCATT1586500.010.85914392-93
TGGTCGC5064400.010.79435390-91
Produced by FastQC (version 0.11.5)