FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86735323
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA2691650.019.1855394-95
ATTAAAA2331500.016.8502294-95
TCATTAA3155900.016.6341594-95
TTAAAAA2004500.015.81122494-95
TATACCG231950.015.3175015
GCCGTAT7717450.015.12956294-95
CGCCGTA9302000.014.63517794-95
CCGTATC6177700.014.53367394-95
ATCATTA3552050.014.25546492-93
GTCTAGG237200.013.5630791
CGTATCA5135050.013.33978194-95
TAGACGG122500.013.0669595
TATCATT3927850.012.92179392-93
TAGAGCG234100.012.3362425
TCGCCGT11086600.011.76802894-95
AGTACCG222850.011.6801565
GTACCGT182500.011.6599486
CCTAGAC242850.011.6376173
GTCTATG282100.011.5896321
GTATCAT4433500.011.58945894-95
Produced by FastQC (version 0.11.5)