FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111241228
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG116150.025.0272435
TATACCG298200.022.1405285
GTCTAGG301700.021.6888731
GTACCGT172650.021.4864866
ATCATTA3358400.021.18884594-95
TCATTAA2585750.020.90268194-95
AGTACCG238200.020.3394495
TATCATT4214700.019.7819594-95
TACCGTG215000.019.507377
GTATCAT5075900.019.07904494-95
TACACCG223000.018.9994375
CATTAAA1890100.018.78498394-95
GTCTATG345450.018.680691
CACTGAG370550.018.4839557
TAGAGCG262700.018.4063855
ACTGAGC345500.018.2293668
ACTGTCC183550.018.1659818
CTAGACA324200.017.962184
CGTATCA6230550.017.9306294-95
CCTAGAC311450.017.8587913
Produced by FastQC (version 0.11.5)