FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91214979
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG92100.027.1273575
TATACCG287200.022.5916335
GTCTAGG256450.022.273521
TCATTAA2423650.022.06562294-95
ATCATTA3165200.021.9382994-95
GTACCGT138400.021.5528836
CATTAAA1714600.020.35046694-95
TATCATT3921500.020.06860594-95
AGTACCG205650.018.8701235
GGACTGC184900.018.4446056
GTATCAT4616400.018.21502194-95
ACTGTCC141400.018.20658
GACGGTC122500.017.9911737
CCTAGAC261800.017.9617883
GTCTATG310200.017.8166071
TAGAGCG254200.017.5644155
TACCGTG182600.017.3241127
GTATAAG440350.017.1911491
CTAGACA282300.017.1284684
CACTGAG351250.017.1196527
Produced by FastQC (version 0.11.5)