FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107574946
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG111400.025.5400755
TCATTAA3852900.023.2280694-95
CATTAAA2760200.022.25325494-95
GTACCGT156850.021.9853446
TATACCG302250.021.9067045
ATCATTA4940700.021.68785394-95
GTCTAGG296150.021.5179711
AGTACCG230000.019.6189755
TACCGTG200750.019.5670387
GGACTGC210050.019.062776
TATCATT5984500.018.96193194-95
CCTAGAC299150.018.466083
GTCTATG346700.018.0790351
CACTGAG355150.017.854377
CTAGACA315150.017.5736774
TACACCG202300.017.468585
ACTGTCC160600.017.331158
AAGACCG238850.017.0624965
ACTGAGC329800.017.0232378
GTGTATG399200.017.0108471
Produced by FastQC (version 0.11.5)