FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_CUI15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_CUI15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115153877
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3605950.023.28089194-95
TAGACGG124400.022.6419895
ATCATTA4691400.022.43176894-95
CATTAAA2533600.021.90098294-95
GTCTAGG305300.020.59311
TATCATT5834350.020.03280694-95
TATACCG316750.019.8991535
GTACCGT172350.019.7876226
GTATCAT6957350.018.1339894-95
AGTACCG241250.017.5424655
GGACTGC235000.017.4835136
TACCGTG217500.017.3394647
ACTGTCC177900.017.3010838
CCTAGAC315750.016.9837553
CACTGAG385300.016.790077
GTCTATG356000.016.619121
TAGAGCG285850.016.500285
CTAGACA333900.016.103154
TACACCG225400.016.0576255
ACTGAGC361700.016.0471138