FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93063439
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3445850.025.555694-95
CATTAAA2366250.024.68141294-95
ATCATTA4640700.023.80169794-95
TAGACGG108750.021.0959475
TATCATT5866850.021.06703894-95
TATACCG222000.021.0106585
GTCTAGG227600.019.9397751
GTACCGT155550.019.6040886
GTATCAT7195400.018.67936994-95
AGTACCG210250.017.869885
GTCTATG260200.017.7155061
GTGTATG293250.017.3556391
TAGAGCG231100.017.264765
TACACCG186850.016.5743185
TACCGTG198600.016.574117
ATTAAAA1854200.016.5536994-95
GTATAAG326700.016.4950281
CGTATCA8674050.016.237494-95
CACTGAG338350.016.1859937
CTAGACA247700.016.1652874
Produced by FastQC (version 0.11.5)