FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94797826
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1219970.12869176978805402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3234750.023.77625594-95
ATCATTA4287000.022.74618594-95
CATTAAA2305800.022.55570294-95
TATCATT5347800.020.50798894-95
GTATCAT6514200.018.20930394-95
TATACCG229450.017.326785
GTCTAGG230550.017.0461081
TAGACGG111400.016.5434865
GTACCGT157950.016.0884846
CGTATCA7833700.016.05347894-95
ACTGTCC163250.015.7987678
GTCTATG269150.015.166441
GTATAAG342100.014.9327661
CCTAGAC237800.014.8209323
ATTAAAA1932150.014.79800994-95
AGTACCG221150.014.712425
TACCGTG201000.014.4857597
CCGTATC9251650.014.48432994-95
GTCTTAG229700.013.8611311
CTAGACA265850.013.7216234
Produced by FastQC (version 0.11.5)