FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69285670
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2221860.32068102971364787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC1714000.016.47357694-95
CGTATCA1398750.015.57657594-95
GCCGTAT2286700.015.5570494-95
CGCCGTA2856800.014.38866394-95
GTATCAT1232250.013.25998394-95
TCATTAA879100.013.1187994-95
CATTAAA800750.012.83048194-95
GTCTAGG197200.012.0245931
TATACCG197000.011.9107575
TAGAGCG233850.011.3947325
ATCATTA986100.011.30512994-95
TATCATT1116200.010.89810394-95
GTATAAG215300.010.85921
CCTAGAC196050.010.7813563
TCGCCGT3726600.010.68998894-95
TATACTG249200.010.6547295
TGGTCGC3955050.010.53974790-91
GTATAGG248150.010.5131991
TAGACGG126900.010.4054735
ACTGTCC192450.010.143778
Produced by FastQC (version 0.11.5)