FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64693991
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1096140.16943459246470047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA1377550.019.17639594-95
ATCATTA1693950.018.35374394-95
CATTAAA1096900.017.550694-95
TATCATT2049950.016.98180694-95
GTATCAT2419400.016.91827294-95
CGTATCA2896250.016.72071594-95
CCGTATC3516450.016.08619394-95
GCCGTAT4320550.014.84807394-95
TATACCG165400.013.6981965
CGCCGTA5195000.013.61052594-95
GTCTAGG171000.012.7834011
GTATAGG219150.012.2732511
TAGACGG105100.012.2474925
GTATAAG214550.011.5839811
CCTAGAC167650.011.4745153
GTCTATG207050.011.4068531
GTACCGT139750.011.1481446
TATACTG248350.011.0355045
TCGCCGT6304550.011.0177994-95
GTGTATG258750.010.9642431
Produced by FastQC (version 0.11.5)