FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109206034
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2265260.20742993010807445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2633500.020.74469894-95
TCATTAA2045100.020.59783694-95
TATCATT3257000.019.40217694-95
GTATCAT3940100.018.60256494-95
CATTAAA1540950.018.33755194-95
TAGACGG145050.018.0432745
GTCTAGG278050.018.0309451
TATACCG270150.017.8636515
CGTATCA4814000.017.65086294-95
CCGTATC5904300.016.9086594-95
GTCTATG322300.016.4105741
GTACCGT203900.015.8872616
GCCGTAT7446750.015.74056594-95
CACTGAG424150.015.4426387
ACTGTCC208500.015.2632298
CCTAGAC273700.014.9941963
ACTGAGC430450.014.9297588
CGCCGTA9099550.014.85701194-95
AGTACCG274450.014.6935225
TATACTG379650.014.6881185
Produced by FastQC (version 0.11.5)