FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91320822
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1500170.16427469301579437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2192350.021.89001394-95
TCATTAA1661850.021.4221994-95
TAGACGG114200.020.6713815
TATCATT2752150.020.4681494-95
TATACCG236000.020.186835
GTCTAGG231000.019.9132271
GTATCAT3349600.019.37337994-95
CATTAAA1204900.018.86121494-95
GTACCGT159100.018.2112226
CGTATCA4099350.017.96614394-95
GTCTATG277800.017.3795991
AGTACCG226600.016.7481215
CCGTATC4958500.016.70246594-95
CCTAGAC238950.016.4789583
GTATAAG342900.016.2835351
GTGTATG325050.016.1397631
TATACTG339900.015.9166535
ACTGTCC166650.015.7329148
CTAGACA280000.015.7253964
TAGAGCG238100.015.5601725
Produced by FastQC (version 0.11.5)