FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116340858
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1612990.13864346780045236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA6070950.026.8586894-95
CATTAAA4129750.026.56398294-95
ATCATTA8275300.024.69001894-95
TATCATT10580500.021.72236894-95
TATACCG220150.021.186935
GTACCGT154750.020.4111086
GTCTAGG244850.020.2035061
GTATCAT13129100.018.93577694-95
ATTAAAA3071500.018.81616294-95
CTAGACA260750.017.8334084
TACCGTG197950.017.78017
TAGACGG118350.017.7791375
ACTGTCC166200.017.6330158
AGTACCG216650.017.2759825
CCTAGAC263000.016.6876223
ATACCGT252000.016.6808556
GTCTATG268800.016.670881
TACACCG204600.016.6219755
GTCTTAG261250.016.3705141
TAGAGCG243250.016.3045525
Produced by FastQC (version 0.11.5)