FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n02_AZU15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n02_AZU15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70838246
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6394780.9027298614931827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG356100.013.5314481
TATACTG152300.012.1631095
TATTGGG398300.011.7390022
GTATAGG191650.011.7281811
CTATACT199400.010.5526124
TAGACTG134800.010.3594665
GGACCGT304200.010.3054026
GTCTAGG185600.010.0621991
TAGGACC228450.010.0216064
TTAGGAC220350.09.9804683
ACTGAGC389600.09.9604528
TCTCGGT1200600.09.75019594-95
CCTATAC193850.09.7031423
TATACAG191850.09.6804355
GTGTAGG291000.09.667341
TGGACAG398800.09.6116585
GTCTTAG122300.09.5972941
GTATTAG173200.09.5479391
GTCTATG203400.09.5320771
TAGTACC206550.09.5204314
Produced by FastQC (version 0.11.5)