FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68660140
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG112050.017.8710351
TAGACGG46100.016.4697825
GTACCGT60650.016.0395376
AGTACCG84600.015.6495785
GTGTATG123900.015.4708161
GTATAGG130800.014.800151
ACTGTCC70600.014.716688
CACTGAG134550.014.7035687
TACCGTG75200.014.69977
TAAGGTG109000.013.4088955
GTCCTGG86850.013.3628611
GTCTTAG84850.013.117271
TATACTG156300.013.0550425
TCTTCTG2415450.013.00651694-95
GACAGTG76900.012.894087
TAGAGGT104200.012.7967164
GTCCTAA107000.012.7578521
TACACCG93950.012.6778535
TTCTGCT1987150.012.4812994-95
GCATAGG90750.012.3692921