FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_SAL60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_SAL60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103239169
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2676000.024.99186194-95
CTTCTGC3882900.023.42642294-95
TCTGCTT2067200.022.79925594-95
TAGACGG101150.022.3061775
TCTTCTG5020650.020.48756494-95
GTCTAGG252900.019.7686251
TATACCG267850.019.3300175
GTACCGT152350.018.9253396
GTCTTCT6061500.018.38945294-95
CTGCTTG1230600.017.98089494-95
AGTACCG209000.017.3183355
ACTGTCC154550.016.9954768
TAGAGCG245250.016.5597785
TACCGTG197800.016.5211167
GTATAAG425600.016.4747351
TACACCG202100.016.3818865
GTCTATG297700.016.3303181
CACTGAG339500.016.1871667
CCTAGAC280400.016.1784273
AAGACCG226650.016.0744865