FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_SAL15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_SAL15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69123653
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2539800.025.4548994-95
TAGACGG68400.025.0696685
TCTGCTT1791250.023.7910694-95
CTTCTGC3461500.023.49200894-95
GTACCGT97600.021.5601336
GTCTAGG173550.020.7470191
TATACCG185450.020.4140425
TCTTCTG4578350.020.29636694-95
CTGCTTG1112650.020.16229894-95
AGTACCG142950.018.907135
TACCGTG128550.018.733317
GGACTGC134400.018.6962246
ACTGTCC103750.018.6788758
GTCTTCT5484650.017.89689494-95
CCTAGAC195600.017.6065733
GTGTATG236600.017.3492411
GTCTATG205000.017.123281
TAGAGCG164850.017.0868995
GTATAAG304650.017.0492741
CTAGACA195900.016.8033664
Produced by FastQC (version 0.11.5)