FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_POK60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_POK60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87070649
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG90500.026.5580775
TTCTGCT2717200.023.98628494-95
GTCTAGG220150.022.6217461
CTTCTGC3658000.022.53208294-95
TATACCG239450.022.19785
TCTGCTT1976500.021.90978494-95
GTACCGT137550.021.5831176
AGTACCG193650.020.1872445
TCTTCTG4825050.019.7524294-95
CCTAGAC239800.019.0558833
TAGAGCG218650.019.0087385
ACTGTCC139000.018.7943218
CTGCTTG1275400.018.72915594-95
ACTGAGC280400.018.514888
GTGTATG305250.018.449911
GTATAAG386400.018.4158881
CACTGAG297500.018.4086137
GTCTATG265550.018.2884791
TACCGTG177150.018.2325127
GGACTGC181450.017.9319826
Produced by FastQC (version 0.11.5)