FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_POK5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_POK5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83013270
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA814750.016.59187194-95
TGCTTGA1017600.016.34646694-95
TCTGCTT1701700.016.06375994-95
GCCGTCT5426800.015.81346794-95
TTCTGCT1989500.015.64758694-95
CCGTCTT4367700.015.58506394-95
CTGCTTG1323150.015.13658494-95
TGCCGTC6575000.015.11264194-95
TATACCG254950.014.4951835
CGTCTTC3810500.014.43045994-95
TAGACGG119750.014.2799295
GTCTAGG221800.013.6603741
GTCTTCT3118100.013.45636294-95
ATGCCGT7325250.013.11933894-95
GTATAGG359500.012.9397131
CTTCTGC2306750.012.45775294-95
ACTGTCC199150.012.3069068
TATGCCG8113100.012.22667692-93
TAGAGCG256450.012.1877165
CCTAGAC226500.011.9331243
Produced by FastQC (version 0.11.5)