FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_POK180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_POK180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87271493
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG94500.027.092815
GTCTAGG221300.022.334061
TATACCG242300.020.976235
CTTCTGC2255350.020.82666694-95
TTCTGCT1709750.020.63020994-95
GTACCGT148300.019.4421636
AGTACCG195250.019.1947775
TCTTCTG2988200.019.03633794-95
GTATAAG388900.018.898361
ACTGTCC140000.018.7957088
CACTGAG314000.018.5908477
ACTGAGC309500.018.523528
GTCTTCT3616950.018.33654694-95
TAGAGCG218600.018.2743915
CCTAGAC241550.018.269093
GGACTGC191850.017.9751246
GTCTATG273150.017.7984941
GTCTAGA230600.017.575751
TCTGCTT1333400.017.4994194-95
GTGTATG314750.017.4864311
Produced by FastQC (version 0.11.5)