FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_POK15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_POK15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106947007
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT5552800.026.50718394-95
TCTGCTT3791500.025.68238894-95
TAGACGG91650.025.4476645
CTTCTGC7848300.024.73101894-95
GTACCGT134050.023.599696
GTCTAGG230200.022.5436521
TATACCG222800.022.15135
CTGCTTG2425150.021.92249394-95
TCTTCTG10595850.021.85954594-95
ACTGTCC139250.021.5234818
TACCGTG177100.020.624637
TAGAGCG232450.020.1486635
AGTACCG191100.019.6614115
GTCTATG260000.019.5207211
AAGACCG203100.019.5054055
GTCTTCT13032800.019.14793694-95
GACAGTG143200.019.0059687
TACACCG170450.018.9779365
CACTGAG310150.018.9135727
ACTGAGC303850.018.836768
Produced by FastQC (version 0.11.5)