FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108002092
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG108400.030.5423985
TTCTGCT3099150.024.83176694-95
CTTCTGC4163100.023.06591494-95
GTACCGT157550.022.9438296
GTCTAGG304900.022.7155231
TCTGCTT2262350.022.50589694-95
GACGGTC130900.022.315847
TATACCG347600.020.6483025
GGACTGC214650.020.181976
AGTACCG236450.019.968565
TACCGTG215800.019.9193367
CCTAGAC325150.019.6494063
TCTTCTG5467850.019.58244994-95
ACTGTCC161150.019.1584668
CTGCTTG1385350.019.15239194-95
GTGTATG397650.019.0680431
GTCTATG352450.019.030071
GCCCTAG158650.018.7994981
CCGTGCG162300.018.5837339
CTAGACA329650.018.5449284