FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52666310
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT810800.015.78516194-95
GCCGTCT736150.014.76621494-95
TATACCG160600.013.427725
TGCCGTC1114450.013.4128494-95
TTCCGTC715900.013.36262194-95
TAGACGG77150.013.3602875
TCCGTCT534850.013.32121494-95
GTCTAGG163200.012.5589831
CCTAGAC161100.012.3836593
GTACCGT120550.012.2936096
TAGAGCG162000.012.2854475
CGTCTTC758300.012.22083294-95
AGTACCG141600.011.6736865
ACTGAGC230600.011.4936028
ATGCCGT868800.011.328032594-95
TATGCCG941950.011.2601592-93
CTAGACA226200.011.2554844
GTCTATG187850.011.2400731
GTCTAGA181650.011.0739451
ACTGTCC135550.011.0030078