FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86735323
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1246600.020.09914894-95
TGCTTGA1658950.019.53119994-95
CTGCTTG2173200.018.24042594-95
TCTGCTT2833950.018.21963394-95
TTCTGCT3291700.016.16793494-95
TAGACGG123850.015.3413275
TATACCG240450.015.27051455
GCCGTCT8042200.015.04159194-95
TGCCGTC9614150.014.41664294-95
GTCTAGG247700.014.152461
CCGTCTT6559300.014.13818494-95
CTTCTGC3757600.013.86238892-93
TAGAGCG243500.013.1284985
CTTGAAA1104900.012.7355594-95
TCTTCTG4202900.012.67280392-93
CGTCTTC5662800.012.55578594-95
CCTAGAC247050.012.3825323
GTACCGT183950.012.2915356
ATGCCGT10737450.012.27188194-95
ACTGAGC370900.012.2555638
Produced by FastQC (version 0.11.5)