FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111241228
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG119450.027.5180025
GTCTAGG299700.023.998311
TATACCG303750.022.3310345
GTACCGT170550.022.1417716
AGTACCG233000.021.5076775
CTTCTGC2646000.021.46953894-95
TTCTGCT1902150.021.08429594-95
CACTGAG374350.020.554917
ACTGAGC345950.020.1965738
TAGAGCG256600.020.1034265
TACCGTG217350.020.0614097
GTCTATG343150.019.5456771
TCTTCTG3454100.019.53865294-95
GTCTTCT4206200.018.89636294-95
TACACCG231750.018.815685
GGACTGC236300.018.7750076
ACTGTCC189450.018.7536328
CCTAGAC322000.018.307183
GTGTATG402800.018.127361
GTATAAG493550.017.916941