FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91214979
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG93950.029.7792515
TTCTGCT2100250.023.78607694-95
GTACCGT145350.023.1373446
CTTCTGC2908400.022.89355394-95
TATACCG275850.021.3006085
GTCTAGG252600.021.1275541
TCTGCTT1573150.020.5782794-95
GACGGTC117700.020.5409477
AGTACCG218500.020.521825
TCTTCTG3820500.020.17371694-95
GTCTATG302550.019.9976831
GGACTGC192550.019.1431546
ACTGTCC142950.018.9395568
TAGAGCG258050.018.5178015
CCTAGAC268750.018.1519973
TACCGTG187500.017.9607337
GTCTTCT4570550.017.94287194-95
GTATAAG444650.017.5862481
CACTGAG366900.017.2827437
GTGTATG342550.017.079331