FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107574946
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG108300.027.5002885
TTCTGCT3755850.024.76781894-95
TCTGCTT2719550.023.16391294-95
GTCTAGG286500.023.0610241
GTACCGT162900.022.5984556
CTTCTGC4987450.022.52160894-95
TATACCG304850.022.157035
GGACTGC215050.021.2266836
AGTACCG234450.020.5035215
CTGCTTG1699850.019.96946194-95
GTCTATG335150.019.7844731
TACCGTG208000.019.7300427
ACTGTCC162850.019.7168038
CACTGAG363750.019.2996317
TAGAGCG260200.019.2959375
CCTAGAC311500.019.2751833
TACACCG211950.019.0943265
CTAGACA320100.019.0091044
TCTTCTG6438600.019.00590394-95
GTGTATG393150.018.6684651
Produced by FastQC (version 0.11.5)